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KMID : 0378019720150010092
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New Medical Journal
1972 Volume.15 No. 1 p.92 ~ p.99
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Effect of alpha-tocopherol on the hepatotoxicity induced with carbon tetrachloride
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Abstract
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The authors have demonstrated the effect of alpha-tocopherol on the hepatotoxicity due to carbon tetrachloride, observing the distribution and alteration of the pyroninophilic granules in the hepatic cells of the mature albino mice of the male treated with alpha-tocopherol and carbon tetrachloride.
68 healthy male mice were divided into 4 groups; 1. the control, 2. the alpha-tocopherol, 3. the carbon tetrachloride, and 4. the alpha-tocopherol plus carbon tetrachloride group. The experimental animals of the alpha-tocopherol and the alpha-tocopherol plus carbon tetrachloride groups were given 200mg./kg. (of a body weight) of alpha-tocopherol after being deprived of food for 6 hours. The alpha-tocopherol, which contains 987mg. of alpha-tocopherol acetate per cubic centimeter, was diluted in the ratio of one part of it to 14 parts of paraffin oil and a single dose of it given by way of the gastric tube was about 0.06 cc. each mature mouse. Carbon tetrachloride was diluted in the ratio of one part of it to 2 parts of paraffin oil and a single dose of it was about 0.06cc. each mature mouse as well.
6 hours after the previous administration of the alpha-tocopherol solution the mice of the alphatocopherol group and the alpha-tocopherol plus carbon tetrachloride group were given a single dose of 0. 06 cc. of paraffin oil and carbon tetrachloride solution respectively. The experimental mice of the control and the carbon tetrachloride groups were given a single dose of 0.06 cc. of paraffin oil after being deprived of food for 6 hours as well. 6 hours after the previous administration of paraffin oil the mice of the control and the carbon tetrachloride groups were given a single dose of 0. 06 cc. of paraffin oil and carbon tetrachloride solution respectively.
After the last administration of paraffin oil or carbon tetrachloride solution the animals of each group were sacrificed in intervals of 1, 2, 3, 6, and 12 hours respectively. Histochemical preparations
were; prepared by way of the methyl green and pyronin method for the RNA granule´ in the hepatic cell and oil red 0 stain for the fat deposit.
hepatotpxicity due to carbon tetrachloride was evident in the hepatic cell by the clump(aggre
gation or agglomeration) formation of the pyroninophilic granules in the cytoplasm of the hepatic cells of the centrolobular area occurring as early as the 3 hour-period. Thereafter a decrease of pyronophilic granules occurred in the carbon tetrachloride group was observed as late as the 12 hour-period.
However, a considerable number of the pyroninophilic granules in the hepatic cells of the alphatocopherol plus carbon tetrachloride group were readily persisted in all zones of the hepatic lobules.
Consequently it is suggested that the decomposition of the pyroninophilic granules, induced with carbon tetrachloride, would be reduced by a previous administration of alpha-tocopherol.
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